Many cellular processes are orchestrated by dynamic changes in the plasma membrane to form membrane projections and endocytic compartments in response to extracellular cue changes. Our previous studies show that post-translational modifications of ACAP4 regulate membrane dynamics and curvature in response to epidermal growth factor and chemokine (C−C motif) ligand 18 stimulation (Zhao et al., 2013; Song et al., 2018). However, there is no quantitative measurement to annotate magnitude of dynamic membrane cytoskeletal remodeling in stimulus-elicited mechanosensation on the plasma membrane.